The formation of conjugation tubes in yeast cells of the jelly fungusSirobasidium magnum(Basidiomycotina) was shown to be mediated by diffusible pheromones which passed through dialysis membranes of 12ensp;000 to 14ensp;000 daltons molecular exclusion limit. The yeast cells were responsive to pheromone only in G1of the cell division cycle. Although caffeine induced morphological abnormalities in the yeast cells and although conjugation tube formation was catabolically repressed by glucose, evidence for the involvement of cyclic nucleotides as secondary messengers in conjugation tube induction was negative. There was some evidence for the presence of a conjugation inhibitor(s).
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