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Multiplex Targeting,Tracking,and Imaging of Apoptosis by Fluorescent Surface Enhanced Raman Spectroscopic Dots

机译:荧光表面增强拉曼光谱点对细胞凋亡的多重靶向,跟踪和成像

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摘要

We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material(F-SERS dots)composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Raman labels for multiplex targeting,tracking,and imaging of cellular/molecular events in the living organism.In this study,F-SERS dots fabricated with specific target antibodies(BAX and BAD)were employed for the detection of apoptosis.The F-SERS dots did not show any particular toxicity in several cell lines.The F-SERS dots could monitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals in both cells and tissues with high selectivity.Our results clearly demonstrate that F-SERS dots can be easily applicable to multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.
机译:我们已经开发了多功能的荧光表面增强拉曼光谱标记材料(F-SERS点),该材料由嵌入银纳米粒子的二氧化硅球与荧光有机染料和特定的拉曼标记组成,用于生物体内细胞/分子事件的多重靶向,跟踪和成像本研究采用由特异性靶标抗体(BAX和BAD)制备的F-SERS点检测细胞凋亡.F-SERS点在几种细胞系中均未显示任何特异性毒性。通过荧光图像以及细胞和组织中的拉曼信号,细胞凋亡具有高效且同时的高选择性。我们的结果清楚地表明,F-SERS点可轻松应用于对维持细胞稳态至关重要的各种细胞/分子事件的多重分析。

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