A Nanoparticle-Based Model Delivery System To Guide the Rational Design of Gene Delivery to the Liver.1.Synthesis and Characterization

摘要

Nonviral gene delivery systems are amenable to forming colloidal particles with a wide range of physicochemical properties that include size,surface charge,and density and type of ligand presented.However,it is not known how to best design these particles without having a set of physicochemical design constraints that have been optimized for the intended gene delivery application.Here,a nanoparticle-based model delivery system is developed that can mimic the surface properties of nonviral gene delivery particles,and this model system is used to define design constraints that should be applied to next generation gene delivery particles.As a test case,a well-defined nanoparticle-based system is developed to guide the rational design of gene delivery to hepatocytes in the liver.The synthetic scheme utilizes monodisperse polystyrene particles and provides for variation of mean particle size and particle size distribution through variation in reaction conditions.The nanoparticles are PEGylated to provide stability in serum and also incorporate targeting ligands,e.g.,galactose,at tunable densities.Four nanoparticles are synthesized from uniformly sized polystyrene beads specifically for the purpose of identifying design constraints to guide next generation gene delivery to the liver.These four nanoparticles are Gal-50 and Gal-140,that are galactosylated 50 and 140 nm nanoparticles,and MeO-50 and MeO-140,that are methoxy-terminated 50 and 140 nm nanoparticles.All four particles have the same surface charge,and Gal-50 and Gal-140 have the same surface galactose density.The availability of galactose ligands to receptor binding is demonstrated here by agglutination with RCA_(120).