The aim of this study was to investigate the effect of 400?ml whole blood donation on the human electroencephalogram (EEG) and hematology, not earlier reported in the literature. EEG activity was recorded from ten male blood donors (experiment group) before, during and after blood donation (i.e., 400?ml whole blood withdrawal). EEG topography and regional spectral field powers analyses were carried out via fast Fourier transformation. The venous hemoglobin (Hb) concentration was measured with a hematology analyzer. In the control investigation, 12 male age-matched volunteers (control group) were kept in semi-sitting position for the duration of a blood donation without actually vena puncture. The volunteers had no prior experiences of blood donation. Within the experiment group, post-donation Hb concentration decreased by 3.7% compared with the pre-donation Hb values (P?
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