Effect of varying high temperatures during reproductive growth on reproductive function, oxidative stress and seed yield in chickpea genotypes differing in heat sensitivity.

摘要

The mechanisms affecting the heat sensitivity of chickpea are largely unknown. Heat-tolerant (ICCV07110, ICCV92944) and heat-sensitive (ICC14183, ICC5912) chickpea genotypes were sown in February in the soil-filled pots. At the time of flowering, these were subjected to varying dayight temperatures of 30/20, 35/25, 40/30 and 45/35 degrees C in the growth chambers (12 h light/12 h dark; light intensity, 250 micro mol m-2 s-1, 80% relative humidity). The pollen viability, pollen germination, tube growth, pollen load and stigma receptivity decreased with increases in temperatures to 45/35 degrees C. The heat-tolerant genotypes experienced significantly less damage to pollen and stigma function. Membrane integrity, chlorophyll content, photochemical efficiency and cellular oxidizing ability were inhibited by the increase in temperature, with greater impacts on the sensitive genotypes. Oxidative injury as lipid peroxidation and hydrogen peroxide content was significantly greater in sensitive genotypes at 40/30 and 45/35 degrees C. Enzymatic and non-enzymatic antioxidants showed increased levels at 40/30 degrees C, but decreased considerably at 45/35 degrees C. Heat-tolerant genotypes possessed greater activity of ascorbate peroxidase and glutathione reductase, along with higher levels of ascorbate and reduced glutathione at 40/30 and 45/35 degrees C. Biomass, pod set and yield were not affected significantly at 35/25 degrees C, but began to decrease at 40/30 degrees C and were lowest at 45/35 degrees C. The sensitive genotypes were not able to set any pods at 45/35 degrees C, whereas the tolerant genotypes produced only few fertile pods at this temperature. It was concluded that heat stress leads to loss of pollen as well as stigma function and induces oxidative stress in the leaves that cause failure of fertilization and damage to the leaves, respectively.