Sensitive amperometric pyridoxine sensor based on self-assembled Prussian blue nanoparticle-modified poly(o-phenylenediamine)/glassy carbon electrode

摘要

Prussian blue nanoparticles (PBNPs) were prepared by a self-assembly process on a glassy carbon electrode (GCE) modified with poly(o-phenylenediamine) (PoPD) film. The stepwise fabrication process of PBNP-modified PoPD/GCE was characterized using scanning electron microscopy and electrochemical impedance spectroscopy. The prepared PBNPs showed an average size of 70 nm and a homogeneous distribution on the surface of the modified electrode. The PBNPs/PoPD/GCE showed electrocatalytic activity towards the oxidation of pyridoxine (PN) and was used as an amperometric sensor. The modified electrode exhibited a linear response for PN oxidation over the concentration range 3-38.5 M with a detection limit of ca 6.10x10(-7) M (S/N=3) and sensitivity of 2.79936x10(3) mAM(-1)cm(-2) using an amperometric method. The mechanism and kinetics of the catalytic oxidation reaction of PN were investigated using cyclic voltammetry and chronoamperometry. The values of , k(cat) and D were estimated as 0.36, 1.089x10(2) M(-1)s(-1) and 8.9x10(-5) cm(2)s(-1), respectively. This sensor also exhibited good anti-interference and selectivity. Copyright (c) 2016 John Wiley & Sons, Ltd.