Study on Methane Fermentation and Production of Vitamin B_(12) from Alcohol Waste Slurry

摘要

We studied biogas fermentation from alcohol waste fluid to evaluate the anaerobic digestion process and the production of vitamin B_(12) as a byproduct.Anaerobic digestion using acclimated methanogens was performed using the continuously stirred tank reactor (CSTR) and fixed-bed reactor packed with rock wool as carrier material at 55 deg C.We also studied the effects of metal ions added to the culture broth on methane and vitamin B_(12) formation.Vitamin B_(12) production was 2.92 mg/L in the broth of the fixed-bed reactor,twice that of the CSTR.The optimum concentrations of trace metal ions added to the culture liquid for methane and vitamin B_(12) production were 1.0 and 8 mL/L for the CSTR and fixed-bed reactor,respectively.Furthermore,an effective method for extracting and purifying vitamin B_(12) from digested fluid was developed.