Fiber-Optic Infrared Reflection Absorption Spectroscopy for Trace Analysis on Surfaces of Varying Roughness. Part II: Acetaminophen on Stainless Steel

摘要

Investigations of the effects of surface roughness on the utility of grazing-angle Fourier transform infrared reflection absorption spectroscopy (IRRAS) as a method for quantifying trace contamination of metal surfaces have been extended to acetaminophen, a model active pharmaceutical agent, on 316 stainless steel. The effects are more complicated than for the surfactant sodium dodecyl sulfate (SDS) on stainless steel; they include a strong surface-finish dependence of sensitivity and nonlinear behavior at surface loadings above approx1-2 (mu)g cm~(-2). Using data from samples in the loading range 0-0.5 (mu)g cm~(-2), unbiased partial least squared calibrations can be readily achieved for individual surface finishes with detection limits of L_(D) approx= 0.15 (mu)g cm~(-2). However, as found for SDS on stainless steel, models built using data from samples of mixed surface roughness are more problematic.