Cryopreservation of Atlantic cod (Gadus morhua) sperm in large-volume straws: applications for commercial production and gene banking.

摘要

In our study, we used a full factorial analysis of variance design to examine the effects of diluent [Mounib's sucrose-based diluent+hen's egg yolk (EY) and Hanks' balanced salt solution (HBSS)+EY], freezing rate (-2.5, -5.0 and -7.5 degrees C min-1) and thawing rate (2.5, 5.0 and 7.5 degrees C min-1) on motility and velocity of Atlantic cod sperm cryopreserved in 2.5 mL cryogenic straws. We found that post-thaw sperm performance was strongly influenced by the presence of higher-order interactions of the factors we tested. For all models broken down by diluent, the 2.5 degrees C min-1 thawing rate had the lowest sperm motility recovery index. Mounib's sucrose-based diluent+EY had the highest motility recovery index at all thawing rates. Mean per cent motility for fresh sperm (87.7+or-2.9%) was not significantly different than of sperm cryopreserved using Mounib's sucrose-based diluent+EY, frozen at -2.5 degrees C min-1 and thawed at 5.0 degrees C min-1 (77.1+or-2.9%). For Mounib's sucrose-based diluent+EY, velocity was significantly higher with sperm thawed at 7.5 degrees C min-1, than sperm thawed at 2.5 degrees C min-1, while thawing rate had no effect for HBSS+EY. Our findings have implications for cod mariculture and aiding in conservation efforts for a dominant marine fish species.Digital Object Identifier http://dx.doi.org/10.1111/j.1365-2109.2010.02769.x