Circulating immune complexes do not affect microparticle flow cytometry analysis in acute coronary syndrome

摘要

Analysis of shed-membrane microparticles (MPs) remains a challenging issue.Gyorgy et al recently reported in Blood that measuring MP by flow cytometry was flawed because of the overlapping biophysical properties of immune complexes, causing false-positive signals during analysis of synovial fluid from patients with rheumatoid arthritis. They suggested that similar problems might occur during analysis of circulating MP from patients with acute coronary syndrome, where the presence of circulating immune complexes has been reported, therefore raisins a question on the validity of previous reports.