Assessment of the Stability of an Immunoadsorbent for the Extracorporeal Removal of Beta-2-Microglobulin from Blood.

摘要

Background: Dialysis-related amyloidosis (DRA) is a devastating and costly condition that affects patients with end stage kidney disease. A key feature of DRA is the formation of amyloid fibrils, consisting primarily of beta(2)-microglobulin. Except for kidney transplantation, conventional kidney replacement therapies, which are based on nonspecific mechanisms, do not adequately address beta(2)-microglobulin removal. An antihuman beta(2)-microglobulin single-chain variable region antibody fragment (scFv) was developed to confer specificity to beta(2)-microglobulin removal during hemodialysis. Methods: The scFv was immobilized onto agarose and characterized for beta(2)m binding capacity, thermal stability at 37 degrees C, regeneration capacity, storage conditions, and sterility. Results: The beta(2)-microglobulin binding capacity was 1.3 mg/ml scFv gel. The immunoadsorbent is thermally stable, can be regenerated, stored short-term in 20% ethanol, lyophilized for long-term storage, and withstand process conditions similar to that of a patient's hemodialysis therapy. Conclusions: The results support further investigation of immobilized scFvs as a novel tool to remove beta(2)-microglobulin from blood. Copyright (c) 2005 S. Karger AG, Basel.