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Comparison of chemical and fatty acid composition of green coffee bean (Coffea arabica L.) from different geographical origins

机译:不同地理起源的绿色咖啡豆(Coffea Arabica L.)的化学和脂肪酸组成的比较

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摘要

This study aimed to quantitatively screen and compare the chemical compounds including protein, lipid, sucrose, total phenolics (TPC), and total titratable acidity (TTA) along with the fatty acid profile of green coffee beans from various geographical origins (China, Indonesia, Ethiopia, Kenya, Guatemala, Honduras, Brazil, and Colombia). Meanwhile, partial least squares discriminant analysis (PLS-DA) was used to investigate whether the analyzed parameters' data can be successfully applied to differentiate the geographical origins of green coffee beans. The levels of protein, lipid, sucrose, TTA, and TPC in all samples were in the range of 13.06-15.98 g 100 g(-1), 12.88-16.29 g 100 g(-1), 7.30-11.45 g 100 g(-1), 231.43-361.21 mL 0.1 mol L-1 NaOH 100 g(-1), and 37.56-50.57 mg GAE g(-1), respectively. The fatty acid composition was dominated by C-18:2, C-16:0, C-18:1, and C-18:0. The major fatty acid group was unsaturated fatty acids (USFA), accounting for 51.66-54.28% of total fatty acids. Statistical analysis showed significant differences in the chemical and fatty acid composition of green coffee beans from eight geographical origins (P<0.05). Moreover, the PLS-DA results revealed excellent discrimination between the different geographical origins of green coffee beans, highlighting lipid, C-24:0, C-22:0, C-18:3, C-17:0, C-18:0, C-20:0, C-16:0, and protein as discriminatory features.
机译:本研究旨在定量筛选和比较来自不同地理来源(中国、印度尼西亚、埃塞俄比亚、肯尼亚、危地马拉、洪都拉斯、巴西和哥伦比亚)的绿色咖啡豆的化学成分,包括蛋白质、脂质、蔗糖、总酚(TPC)和总可滴定酸度(TTA)以及脂肪酸谱。同时,利用偏最小二乘判别分析(PLS-DA)研究了所分析的参数数据能否成功地用于区分青咖啡豆的地理来源。所有样品中的蛋白质、脂质、蔗糖、TTA和TPC水平分别在13.06-15.98 g 100 g(-1)、12.88-16.29 g 100 g(-1)、7.30-11.45 g 100 g(-1)、231.43-361.21 mL 0.1 mol L-1 NaOH 100 g(-1)和37.56-50.57 mg GAE g(-1)范围内。脂肪酸组成以C-18:2、C-16:0、C-18:1和C-18:0为主。主要脂肪酸组为不饱和脂肪酸(USFA),占总脂肪酸的51.66-54.28%。统计分析显示,来自八个地理来源的青咖啡豆的化学成分和脂肪酸组成存在显著差异(P<0.05)。此外,PLS-DA结果揭示了不同地理来源的青咖啡豆之间的出色区别,突出了脂质、C-24:0、C-22:0、C-18:3、C-17:0、C-18:0、C-20:0、C-16:0和蛋白质作为区别特征。

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