Gene transcription and antioxidants production inArthrospira (Spirulina) platensisgrown under temperature variation

摘要

Aim To investigate the transcription of selected antioxidants and relevant genes under varying temperature conditions, and to identify the optimum temperature for antioxidants production byArthrospira platensis. Methods and Results The dry weight (DW), pigment production, antioxidants production and gene transcription were examined inA. platensisgrowing under three temperatures of 23, 30 and 37 degrees C. The cyanobacterial DW was highest in the high temperatures (30 and 37 degrees C), while the pigments, such as Chl a, carotenoids, C-phycocyanin and total phycobiliprotein contents, showed their maximum value at 30 degrees C. The total soluble protein and carbohydrate contents were highest at 30 degrees C. Lipid peroxidation, as a marker for thermal stress, was high at 23 degrees C, while higher temperatures remarkably reduced lipid peroxidation levels. Antioxidants activity was increased by 1 center dot 5-fold at 30 degrees C and temperature fluctuations induced the antioxidant enzyme activities. The transcriptional abundance of heat shock protein (HSP90), glutamate synthase (GOGAT), delta-9 desaturase (desC), iron-superoxide dismutase (FeSOD) and the large subunit of Rubisco (rbcL) genes was measured under the same temperatures. Conclusion The optimal temperature for growth, biochemical constituents and antioxidants ofA. platensisis 30 degrees C while some antioxidant enzyme activity increased at lower and higher temperatures. Significance and Impact of the Study The study showed the significance of temperature for growth, enzymatic and non-enzymatic antioxidants and gene expression inA. platensis. This contributes to the knowledge of culturingA. platensisto harvest specific antioxidants or as an antioxidant-rich food source.