In this paper, a high performance liquid chromatography technique is established for quantification of paraquat in blood. Samples were pretreated by mixing with hydrochloric acid for precipitation of protein, followed by acetonitrile extraction, ultrasonication, centrifugation, and filtration. In addition, with sodium heptane-acetonitrile-water (1.82 g/50 mL/450 mL) buffer solution as the mobile phase and a C_(18) column as the stationary phase, liquid chromatography separation and determination was carried out on samples of paraquat using a variable wavelength UV detector. The linear range was 0.3-30 ng mL~(-1) (R = 0.9999) with a minimum detection limit for paraquat of 0.026 ng mL~(-1) (S//V >3), and limit of quantification of 0.08 ng mL~(-1). Moreover, the method proposed here is sensitive and accurate, and should have a bright future in numerous practical applications:
展开▼
机译：本文建立了一种高效液相色谱技术，用于定量血液中的百草枯。通过与盐酸混合以沉淀蛋白质来预处理样品，然后进行乙腈提取，超声处理，离心和过滤。另外，以庚烷-乙腈-水（1.82 g / 50 mL / 450 mL）缓冲溶液为流动相，并以C_（18）色谱柱为固定相，对百草枯样品进行了液相色谱分离和测定使用可变波长紫外线检测器。线性范围为0.3-30 ng mL〜（-1）（R = 0.9999），百草枯的最低检出限为0.026 ng mL〜（-1）（S // V> 3），定量限为0.08 ng mL〜（-1）。而且，这里提出的方法灵敏且准确，在许多实际应用中应该有光明的前景：
展开▼
