Interaction of vancomycin with DNAf and determination of DNA via resonance Rayleigh scattering and resonance nonlinear scattering

摘要

Assays were developed for DNA based on enhanced resonance Rayleigh scattering and resonance nonlinear scattering (including second order scattering and frequency doubling scattering) that resul from the interaction of vancomycin with DNA. Vancomycin possesses the unusual property ot promoting the aggregation of DNA, which was concluded from the results obtained with RRS and absorption spectroscopy and from atomic force microscopy. The types of interaction and reasons ben.nd RRS enhancement are discussed. Linear relationships exist over a wide range between the intensity of enhanced scattering and the concentration of DNA. The detection limit (3σ) is in the range of 6.8 to 11.1 ng mL~(-1).