Analysis of volatile compounds of leaves and galls of Schinus polygamus and Baccharis spicata by Headspace Solid-Phase Microextraction

摘要

Headspace Solid-Phase Microextraction (HS-SPME) is presented as an efficient tool to access differences and similarities in the composition of volatiles that might have been caused by insect-plant interaction, using leaves and galls of Schinus polygamus and Baccharis spicata. Method development involved testing nine SPME coatings, resulting in the choice of the triple layer divinylbenzene-carboxen-polydimethylsiloxane (DVB-CAR-PDMS). Qualitative and quantitative differences between distinct plant tissues and also between hydrodistilled oil and headspace chromatographic profile of the same sample confirmed that HS-SPME is a convenient analytical tool to provide information closer to that of the plant in vivo on possible allelochemicals of different plant tissues involved in the insect-plant interaction.