Identification of Geographical Origin of Honeysuckle (Lonicera Japonica Thunb) by Discriminant Analysis Using Rare Earth Elements

摘要

Honeysuckle was collected from four locations in China: Pingyi, Shandong Province; Julu, Heibei Province; Dongkou, Hunan Province; and Fengqiu, Henan Province. The samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) for the rare earth elements La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Sc, and Y to characterize geographical traceability. The accuracy of the entire procedure was verified by the analysis of a citrus leaves standard reference material GBW10020. The classification of honeysuckle was carried out based on rare earth elements composition by a radar plot and linear discriminant analysis. Honeysuckle from the regions was easily distinguished by radar plots. Furthermore, linear discriminant analysis based on concentrations of rare earth elements provided more than 98% accuracy for predictions using leave-one cross-validation. Thus, rare earth elemental concentrations combined with the use of multivariate statistical techniques allows the evaluation of the geographical origin of honeysuckle. This method provides significant potential in differentiating the geographical origin of various plants.