DETERMINATION OF ACARICIDES IN HONEY BY LIQUID CHROMATOGRAPHY WITH ORDINARY, NARROW BORE, AND MICROBORE COLUMNS

摘要

Narrow-bore and microbore columns packed with octadecylsilane were used to compare their sensitivity and efficiency in the separation of coumaphos, fluvalinate, bromopropylate, and 4,4'-dibromobenzophenone from honey with those of ordinary columns, The best sensitivity for acaricides was accomplished by using a 150 mm x 0.32 mm i,d., 5 mu m Spherisorb ODS-2 capillary column, methanol-water (90:10 v/v) as the mobile phase, and 5 mu L/min as the now rate, Detection limits for individual acaricides using a UV detection range from 0.40 to 0.74 mu g/kg of honey were comparable to those obtained by gas chromatography using an electron capture detector. All acaricides were separated in <12 min, The coefficients of variations on real samples were <6.2%.