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Palladium as a substrate for self-assembled monolayers used in biotechnology

机译:钯作为生物技术中自组装单层膜的基质

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This paper describes self-assembled monolayers (SAMs) on palladium that resist the nonspecific adsorption of proteins and the adhesion of mammalian cells. These SAMs form when thin films of palladium are exposed to solutions of alkanethiol with the general structure HS(CH2)(m)(OCH2CH2)(n)OH (m = 2, 11; n = 3, 6, 7). Ellipsometry and surface plasmon resonance spectroscopy (using a palladium-on-gold substrate) showed that these SAMs resist adsorption of all proteins present in bovine serum. Microislands of SAMs of octadecanethiol on palladium allowed patterned adhesion and growth of mammalian cells (in a "sea" of oligo(ethyleneglycol)terminated SAM). The oligo(ethyleneglycol)-terminated SAM resisted the invasion of cells for over four weeks under standard conditions of cell culture; similar SAMs on gold remained patterned for only two weeks.
机译:本文描述了钯上的自组装单分子膜(SAMs),该膜可抵抗蛋白质的非特异性吸附和哺乳动物细胞的粘附。这些SAM在钯薄膜暴露于具有一般结构HS(CH2)(m)(OCH2CH2)(n)OH(m = 2,11; n = 3,6,7)的链烷硫醇溶液中形成。椭偏和表面等离振子共振光谱法(使用钯金底物)显示,这些SAMs抵抗牛血清中存在的所有蛋白质的吸附。十八烷硫醇的SAM在钯上的微岛使得图案化的粘附和哺乳动物细胞的生长(在以寡聚乙二醇为末端的SAM的“海”中)。在标准的细胞培养条件下,以低聚乙二醇为末端的SAM可以抵抗细胞侵袭超过4周。相似的黄金SAM仅维持了两周的时间。

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