PFM-Like Enzymes Are a Novel Family of Subclass B2 Metallo-beta-Lactamases from Pseudomonas synxantha Belonging to the Pseudomonas fluorescens Complex

摘要

A carbapenem-resistant Pseudomonas synxantha isolate recovered from chicken meat produced the novel carbapenemase PFM-1. That subclass B2 metallo-beta-lactamase shared 71% amino acid identity with beta-lactamase Sfh-1 from Serratia fonticola. The bla(PFM-1) gene was chromosomally located and likely acquired. Variants of PFM-1 sharing 90% to 92% amino acid identity were identified in bacterial species belonging to the Pseudomonas fluorescens complex, including Pseudomonas libanensis (PFM-2) and Pseudomonas fluorescens (PFM-3), highlighting that these species constitute reservoirs of PFM-like encoding genes.