Simultaneous measurements of the molar radioactivity, radiochemical purity and chemical impurity in the [C-11]choline injection using radio-HPLC with a corona-charged aerosol detector

摘要

[C-11]choline has a weak UV absorption chromophore, and it is challenging to detect less than 1 mu g/mL choline using radio-HPLC-UV. In this study, we established an analytical procedure of [C-11]choline using the radio-HPLC coupled with the corona-charged aerosol detector. As a result, we achieved more than 100 GBq/mu mol (over 0.1 mu g/mL of choline) of molar radioactivity at the end of synthesis, over 95% of radiochemical purity, and more than 0.1 mu g/mL of 2-dimethyoaminoethanol as a chemical impurity.