Elimination terminal fixed region screening and high-throughput kinetic determination of aptamer for lipocalin-1 by surface plasmon resonance imaging

摘要

A highly efficient method for eliminating terminal fixed region interference of aptamer with real-time monitoring of the SELEX process was described by silver decahedra nanoparticles probe (Ag-10-A(10-)RP(15)) capture and block the terminal fixed region candidates. A microarray chip was developed by immobilization of target protein (lipocalin-1 (LCN-1)) and control proteins (Human serum albumin (HSA), Bovine serum albumin (BSA) and Holo-transferrin) on the biochip surface. The nucleic acid pool was first incubated with target and then captured by hybridization with Ag-10-A(10)-RP(15). The work allows rapid screening of aptamer elimination fixed-region interference, and the kinetic constants of candidate sequences can be quickly determined using SPRi technology. Eventually, ten aptamers with high affinity and specific for LCN-1 after only fifth-round of selection was acquired. (C) 2018 Published by Elsevier B.V.