Measuring redox-active species in cells and tissues. Focus on 'A case of mistaken identity: are reactive oxygen species actually reactive sulfide species?'

摘要

the generation of reactive oxygen species (ROS) is inevitable for aerobic organisms and generally occurs at a controlled rate, with endogenous mechanisms tightly regulating ROS production and metabolism. Alterations in the balance between oxi-dant production and metabolism can lead to enhanced oxida-tive stress, which promotes oxidative damage to a wide range of biomolecules. The accumulation of oxidized products is associated with aging and the development of pathologies, such as atherosclerosis (11, 14), diabetes mellitus (9), cardiovascular disease (1), and neurodegenerative disorders (12). This perceived importance of ROS generation in disease progression is supported by a myriad of available methods for measurement of various ROS species (13). However, the assays themselves are not without limitations, including a limited ability to identify the specific ROS responsible for a given footprint of biological damage (4).