Highly sensitive amperometric detection of glutamate by glutamic oxidase immobilized Pt nanoparticle decorated multiwalled carbon nanotubes (MWCNTs)/polypyrrole composite

摘要

A highly sensitive and selective glutamate biosensor using glutamate Oxidase (GlUtOx) immobilized platinum nanoparticle (PtNP) decorated multiwall carbon nanotube (MWCNTs)/polypyrrole (PPy) composite on glassy carbon electrodes (GC) is demonstrated. PtNP decorated MWCNTs (Pt-MWCNTs), PPy and Pt-MWCNTs/PPy composite were characterized by Field Emission Scanning Electron Microscope (FESEM), X-ray diffraction (XRD) and Raman analysis to confirm the formation of the nanocomposite. The glutamate Oxidase (GlUtOx) was immobilized on a GC/Pt-MWCNTs/PPy and characterized by the cyclic voltammetry (CV) and impedance spectroscopy (EIS) analysis. The fabricated L-glutamate biosensor exhibited high sensitivity (723.08 A cm(-2) mM(-1)) with less response time (3 s) with a detection limit of 0.88 mu M. The dynamic range from 10 to 100 mu M with a correlation coefficient (R2) of 0.985 was observed for the L-glutamate biosensor. The analytical recovery of added L-glutamate acid (50 and 100 mu M) in human serum soup were 96.1% and 97.5% respectively. The enzyme immobilized GC/Pt-MWCNTs/PPy/G1UtOx bioelectrode lost 12.6% and 23.8% of its initial activity after 30 days when stored at -20 degrees C and 4 degrees C respectively.