miR-424–5p maybe regulate blood-brain barrier permeability in a model in vitro with Abeta incubated endothelial cells

摘要

The blood-brain barrier (BBB) in AD patients and in animal models is changed. However, the mechanisms are still unclear. Here, we found that miR-424–5p was upregulated in Abeta-incubated microvascular endothelial cells. TEER and HRP exudation tests showed that miR-424–5p silencing significantly decreased BBB permeability in vitro BBB model with Abeta-incubated. MiR-424–5p silencing upregulated expression of the tight junction proteins, ZO-1 and occludin in Abeta-incubated microvascular endothelial cells. Furthermore, dual luciferase reporter gene assay results confirmed the presence of a potential binding site for miR-424–5p on the 3′UTR of Endophilin-1. Endophilin-1 was down-regulated in Abeta-incubated endothelial cells in which miR-424–5p was silenced. In conclusion, the present study demonstrates that miR-424–5p could affect the expression of tight junction proteins (ZO-1 and occludin) via Endophilin-1 and thereby maybe regulate BBB permeability in an BBB model in vitro with Abeta incubated endothelial cells. MiR-424–5p may thus serve as a protective target for AD and provide a new strategy for the prevention and treatment of AD.