Production of 1,3-propanediol using a novel 1,3-propanediol dehydrogenase from isolated Clostridium butyricum and co-biotransformation of whole cells

摘要

Graphical abstractDisplay OmittedHighlights?A newlydhaTgene from isolatedC. butyricumYJH-09 was cloned and expressed.?The mixed whole cells was firstly used for producing 1,3-PD based on a novel characterized PDOR.?The production of 1,3-PD was improved more than 2 times via co-biotransformation.AbstractIn this study, a newly strain namedClostridium butyricumYJH-09 were isolated from the sample of pond soil and identified through physiological, biochemical and 16S rDNA analysis. Then, thedhaTgene encoding a novel 1,3-propanediol dehydrogenase (PDOR) was cloned from this strain and expressed inEscherichia col