Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants

摘要

Simultaneous production of citric acid (CA) and invertase by Yarrowia lipolytica A-101-B56-5 (SUC+ clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. Among the tested substrates the highest concentration of CA was reached from glycerol (57.15g/L) with high yield (Y_(CA/S)=0.6g/g). When sucrose was used, comparable amount of CA was secreted (45g/L) with slightly higher yield (Y_(CA/S)=0.643g/g). In all cultures amount of isocitrate (ICA) was below 2% of total citrates. Considering invertase production, the best carbon source appeared to be sucrose (72380U/L). The highest yield of CA and invertase biosynthesis calculated for 1g of biomass was obtained for cells growing from glycerol (9.9g/g and 4325U/g, respectively). Concentrates of extra- and intracellular invertase of the highest activity were obtained from sucrose as substrate (0.5 and 1.8×106U/L, respectively).