ONE STRAIN-MANY COMPOUNDS METHOD FOR PRODUCTION OF POLYKETIDE METABOLITES USING THE SPONGE-DERIVED FUNGUS Arthrinium arundinis ZSDS1-F3

摘要

Marine-derived fungi are recognized as important sources for a wide array of structurally diverse and pharmacologically active metabolites [1-3]. Recently, we discovered a fungus, Arthrinium arundinis ZSDS1-F3, obtained from the sponge Phakellia fusca Thiele, collected from the Xisha Islands of China in 2012. A. arundinis ZSDS1-F3 was incubated on a rotary shaker at 28°C for 12 days in a liquid medium previously. Detailed chemical investigation resulted in the isolation of cytotoxic pyridine alkaloids arthpyrones A-C [4], cytochalasins arthriniumnins A-D [5], naphthalene derivative 1,8-dihydroxynaphthol-1-O-α-L-rhamnopyranoside, and some known xanthones [6]. Based on the potential biosynthetic ability of this strain, the OSMAC (one strain-many compounds) approach was carried out to find new compounds. This strategy was developed in the early 2000s by Zeeck and co-workers to maximize the productivity of a single microbe, probably through turning on silent or cryptic biosynthetic genes by varying accessible cultivation parameters such as medium composition, pH, temperature, oxygen supply, quality and quantity of tight, bioreactor platform, or the addition of precursors or enzyme inhibitors [7]. One successful application is the isolation of a series of aspochracin-type cyclic tripeptides, sclerotiotides A-K [8], and two novel cyclic hexapeptides sclerotides A and В [9] from Aspergillus sclerotiorum PT06-1 via modification of the culture media and salinity.