Comparative analysis of mice serum proinflammatory cytokine levels following radiation or combined radiation injury

摘要

Male mice (CBA X C578Bl/6)F_1 were used for the experiments throughout this study. Blood serum levels of IL-1#beta#, TNF-#alpha#, IL-3, and Gm-CSF were evaluated by means of enzyme-linked immunosorbent assay at 3, 6, 24, 48 and 72 hours after 7 Gy #gamma#-irradiation alone or combined injury (irradiation + thermal burn). Radiation as well as combined injury did not cause any important alterations of IL-2#beta#, INF-#alpha#, IL-3, and GM-CSF concentrations in the system circulation. Combined injury revealed more enhanced serum levels of IL-6 versus only irradiated mice. A possible significance of this phenomenon at the combined radiation and thermal burns' pathogenesis is discussed.