Study of the possibility of the construction of species-specific antigenic erythrocyte diagnosticum for the detection of antibodies to Rickettsia prowazekii

摘要

Species-specific antigenic erythrocyte diagnosticum (SAED) for the detection of antibodies to R. prowazekii was developed. The cells used as the basis for the preparation of the antigenic erythrocyte diagnosticum were formolated and tanned sheep red blood cells (FT-SRBC). Original indication antigens, solubilized with Triton X-100 from common R. prowazekii antigen for the complement fixation test, the antigen neutralization test and the fluorescent analysis, were used as sensitin. The optimum parameters for sensitizing FT-SRBC were established. The SAED thus obtained was stabilized by the method of lyophilization. In a separate series of experiments the specificity and activity of SAED were studied in comparison with those of preparations obtained with the use of different sensitins. The newly developed SAED was shown to be highly specific.