電子線直接励起蛍光顕微鏡による蛍光ナノダイヤモンドの観察

摘要

We investigated fluorescent nano diamonds as fluorescent markers for a direct electron-beam excitation (D-EXA) optical microscope. The D-EXA microscope excites the nanometric region of the specimen in an atmospheric pressure by electron beam irradiation. We used the fluorescent nano diamonds as fluorescent makers because of their less photobleaching, low cytotoxity, high efficiency of emission. They also can be excited both photoluminescence and cathodoluminescence, so it is possible to demonstrate the correlative observations between the D-EXA microscope and a fluorescent confocal laser microscope. We measured the cathodoluminescent spectra of fluorescent nano diamonds, and presented that the D-EXA microscope can overcome the diffraction limit.