三聚氰胺-铜(Ⅱ)配合物抑制AT-双链铜纳米颗粒合成荧光检测三聚氰胺

摘要

基于三聚氰胺与铜离子配位反应并抑制A T-双链铜纳米颗粒合成,构建了一种新型的"turn-off"策略检测三聚氰胺.当三聚氰胺存在时,与铜离子发生配位反应,使得后期合成铜纳米颗粒的铜离子浓度不够,导致铜纳米颗粒荧光减弱.在最优化实验条件下,对三聚氰胺检测的线性范围为1~150μmol·L-1,检出限达0.5μmol·L-1.此外,该方法还可以检测牛奶样品中的三聚氰胺,回收率良好.%A novel turn-off strategy was developed for the melamine (Mel) detection based on melamine-Cu conjugate and subsequent interrupted the formation of AT-dsDNA copper nanoparticles (AT-dsDNA CuNPs) . The coordination of melamine to copper cannot make the reduction of Cu2+ to Cu0 enough in the AT24-CuNPs synthesis process ,and thereby results in the fluorescence intensity of CuNPs decreasing .Under the optimum conditions ,the concentration of melamine showed a good linear relation with the fluorescence response of CuNPs in the range of 1~150μmol · L -1 .The detection limit was 0.5μmol · L -1 .Furthermore ,good recov-eries were obtained while the proposed method was applied to the analysis of melamine in milk samples .