超短パルスレーザーを用いた先端的分光計測による蛍光タンパク質の発光機構の研究

摘要

Fluorescent proteins such as Aequorea victoria GFP and its mutants are essential as fluorescent markers in living cells in the field of cell and molecular biology. We have investigated the fluorescence mechanism and dynamics of those proteins using ultrafast and nonlinear spectroscopic techniques. In this review, we report our recent works on practically-important fluorescent proteins, which were carried out with femtosecond time-resolved fluorescence spectroscopy and multiplex two-photon absorption spectroscopy.