Grafted Glycopolymer-Based Receptor Mimics on Polymer Support for Selective Adhesion of Bacteria

摘要

A sugar-containing monomer (2-lactobionamidoethyl methacrylate. LAMA) was grafted on a polypropylene (PP) microfiltration membrane surface by UV-induced graft copolymerization. The degree of grafting can be controlled by variation of monomer concentration, UV irradiation time, and phosoinitiator concentration. Fourier transform infrared spectroscopy and scanning electron microscopy were employed to confirm the surface modification on the membranes. The water contact angle was used to evaluate the hydrophilicity change of the membrane surface before and aFter modification. Bacteria capture experiments showed that the membrane could selectively bind E.faecalts while adhesion of S. maltophitia was not influenced by the functionalization of PP with grafted poly(LAMA). The adhesion of E.faecaUs onto poly(LAMA) grafted membrane could be inhibited by 200 mM galactose solution; however, glucose solution showed no inhibition effect. Moreover, occupying sugar residues on the membrane surFace primarily by a galactose targeting lectin, peanut agglutinin, could significantly suppress the following adhesion of E.faecaUs. All these results clearly demonstrate that this poly(LAMA) grafted PP membrane can selectively capture E. faecalis and that this selection is based on the interaction between galactose side groups on grafted flexible functional polymer chains on the membrane surface and galactose binding protein on the E. faecalis cell membrane.