Evaluation of an Automated Solid-Phase Extraction Method Using Positive Pressure

摘要

Investigations for the determination of drugs in real samples, while gaining in importance, are limited by time and sample throughput. In response to this, the authors developed an automated solid-phase extraction (SPE) procedure using a liquid-handling platform with a positive pressure unit. This automated SPE was validated after the manual SPE process. HPLC/MS was used to determine drug concentration. The results showed that 1) automation offers a precise and robust system for sample preparation and 2) automated SPE is suitable for routine analysis with less manual processing and fewer operator errors.