Genetic variants of Mishima cattle based on analysis of mitochondrial DNA D-loop region

摘要

Eighty-nine females of Mishima cattle reared in Mishima Island in 1995 were divided into 6 groups according to maternal lines. In the present study, 2 animals each from 5 lines out of 6 were selected for analysis of the D-loop region of mitochondrialDNA. According to the variants obtained from the analysis, Mishima cattle was assigned into 2 maternal lineages. Maternal lines Takatada, Kamekichi and Yoshimaki were classified as haplotype M1, and Mimatsu and Shiraume were classified as haplotype M2.Thus, 5 maternal lines of Mishima cattle were categorized into 2 lineages, which means that the genetic diversity of Mishima cattle is quite low. The frequency of M2 was only 14.6 percent of all the Mishima cattle in Mishima Island in 1995. It is particulary necessary to take measures to conserve haplotype M2 immediately.