Separation and quantification of carotenoids and chlorophylls in vegetables by high-performance liquid chromatography [Japanese]

摘要

Carotenoids such as beta-carotene, a predominant vitamin A precursor, and lutein and chlorophylls in vegetables have been reported to have antioxidative activity and desmutagenicity. In this paper, we developed a simple and rapid method to measure the carotenoids and the chlorophylls ill vegetables. We adopted a simple extraction method with acetone without Solvent transfer or evaporation for the extraction of them from raw vegetables. When water was added to freeze-dried vegetables before homogenization, pigments were extracted with high yield. beta- and alpha-Carotenes, lutein and chlorophylls-a and -b were separated by HPLC oil all ODS reversed-phase column,using linear gradient elution of ethyl acetate and acetonitrile-water (9 : 1). The contents of them were not reduced when extracts were stored at -20degreesC for two weeks.