Human amnion epithelial cells reduce ventilation-induced preterm lung injury in fetal sheep

摘要

Objective: The objective of the study was to explore whether human amnion epithelial cells (hAECs) can mitigate ventilation-induced lung injury. Study Design: An established in utero ovine model of ventilation-induced lung injury was used. At day 110 of gestation, singleton fetal lambs either had sham in utero ventilation (IUV) (n = 4), 12 hours of IUV alone (n = 4), or 12 hours of IUV and hAEC administration (n = 5). The primary outcome, structural lung injury, was assessed 1 week later. Results: Compared with sham controls, IUV alone was associated with significant lung injury: increased collagen (P =.03), elastin (P =.02), fibrosis (P =.02), and reduced secondary-septal crests (P =.009). This effect of IUV was significantly mitigated by the administration of hAECs: less collagen (P =.03), elastin (P =.04), fibrosis (P =.02), normalized secondary-septal crests (P =.02). The hAECs were immunolocalized within the fetal lung and had differentiated into type I and II alveolar cells. Conclusion: The hAECs mitigate ventilation-induced lung injury and differentiated into alveolar cells in vivo.