Determination of hydrogen sulfide in a yeast culture solution by flow analysis utilising methylene blue spectrophotometric detection

摘要

Gas diffusion and flow injection analysis with a spectrophotometric detector has been developed for the determination of sulfide in a yeast culture solution. A detection limit (signal-to-noise ratio = 3) of 0.2 mu M sulfide was achieved. A relative standard deviation of 3.4% (n = 12) was also achieved for 0.5 mu M sulfide. The technique is highly sensitive, accurate and precise, with low susceptibility to interferences, and allows significant reagent and instrument economy. [References: 11]