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Exploration of Staphylococcus nepalensis(KY024500) Biosurfactant towards Microbial Enhanced Oil Recovery

机译:葡萄球菌(KY024500)生物素质剂探索微生物增强储存的探索

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Abstract > Oleochemicals have long been used as biolubricants, biopolymers, and biosurfactants; an effective alternative to petroleum‐based products. The present study explores the biosurfactant potential of a novel strain, isolated from rocks of earthquake‐prone area. On the basis of morphological, biochemical and 16S rRNA sequencing analysis, the isolate was identified as <fi>Staphylococcus nepalensis</fi> (KY024500). A biosurfactant yield 2.39, 1.39, and 0.9 g?L ?1 was obtained using glycerol, waste orange peel, and diesel as a sole carbon source, respectively. Based on oil recovery experimental findings through sand pack column, the obtained biosurfactant from waste orange peels as a sole carbon source was carried forward for further analysis. Thus, obtained biosurfactant from waste orange peels were subjected to solvent extraction and purified by column chromatography. The purified biosurfactant thus obtained was characterized with the help of fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), gas chromatography‐mass spectroscopy (GC–MS), and MALDI TOF MS/mass spectroscopy (MS) analysis. FTIR spectroscopic analysis revealed the presence of a carbonyl, amine, hydroxyl, and methyl as functional groups. The GC–MS analysis showed the presence of benzene dicarboxylic acid diethyl ester and pthalic acid as fatty acids while MALDI TOF MS/MS analysis shows lysin‐glycin as a hydrophilic dipeptide moiety. This study also demonstrates Microbial Enhanced Oil Recovery (MEOR) potential of the biosurfactant as more efficient than commercial ones. The biosurfactant obtained from waste orange peel as carbon source was able to facilitate a 20% higher recovery of diesel from sand pack recovery column. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译:</span><Abstract Type =“Main”XML:Lang =“en”> <标题类型=“main”>抽象</ title> > 黄油化素长期被用作生物润滑剂,生物聚合物和生物表面活性剂;一种有效的石油型产品替代品。本研究探讨了一种新型菌株的生物活化剂潜力,从地震易发的地区岩石中分离。在形态学,生化和16S rRNA测序分析的基础上,确定了分离物 <fi>金葡萄球菌Nepalensis </ Fi> (KY024500)。生物表面活性剂产率2.39,1.39和0.9g?l ?1 </ sup> 使用甘油,废橙皮和柴油作为唯一碳源获得。基于通过砂包柱的油回收实验结果,将所得生物活性剂从废橙皮中作为唯一碳源进行进一步分析。因此,从废橙皮中获得的生物活性剂经受溶剂萃取并通过柱色谱纯化。由此获得的纯化的生物表面活性剂的特征在于傅立叶变换红外(FTIR),核磁共振(NMR),气相色谱 - 质谱(GC-MS)和MALDI TOF MS /质谱(MS)分析。 FTIR光谱分析显示出羰基,胺,羟基和甲基作为官能团的存在。 GC-MS分析显示存在苯二羧酸二乙基酯和偏苯二甲酸作为脂肪酸,而MALDI TOF MS / MS分析显示赖辛 - 甘氨酸作为亲水偶氮部分。本研究还证明了生物活性剂的微生物增强的溢油(Meor)电位比商业成分更有效。废橙皮作为碳源获得的生物活性剂能够促进柴油包装回收塔的20%柴油恢复。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35863/'>《Journal of Surfactants and Detergents》</a> <b style="margin: 0 2px;">|</b><span>2020年第3期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4818.html" title="表面现象的物理化学">表面现象的物理化学;</a><a href="https://www.zhangqiaokeyan.com/clc/7878.html" title="表面活性剂">表面活性剂;</a><a href="https://www.zhangqiaokeyan.com/clc/1844.html" title="油脂和蜡的化学加工工业、肥皂工业">油脂和蜡的化学加工工业、肥皂工业;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biosurfactant&option=203" rel="nofollow">Biosurfactant;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=S. nepalensis(KY024500)&option=203" rel="nofollow">S. nepalensis(KY024500);</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lipopeptide&option=203" rel="nofollow">Lipopeptide;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MEOR&option=203" rel="nofollow">MEOR;</a> </p> <div class="translation"> 机译:生物活性剂;s。 Nepalensis(KY024500);脂肽;MEOR; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025863888.html">Exploration of Staphylococcus nepalensis(KY024500) Biosurfactant towards Microbial Enhanced Oil Recovery</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth 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