Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection

Páger Csilla; Biherczová Nikoleta; Ligetvári Roland; Berkics Balázs Viktor; Pongrácz Tamás; Sándor Viktor; Bufa Anita; Poór Viktória; Vojs Staňová Andrea; Kilár Ferenc

摘要

Abstract >Capillary isoelectric focusing hyphenated with mass spectrometry detection, following the sequential injection of the carrier ampholytes and the sample zone, is highly efficient for the characterization of proteins. The main advantage of the sequential injection protocol is that ampholytes, with pH ranges, which are not supposed to cover the isoelectric points of the sample components, can be used for separation. The method then allows online mass spectrometry detection of separated analytes either in the absence (substances that have left the pH gradient) or in the presence of low‐level ampholytes (substances that are migrating within the pH gradient). The appearance of the substances within, or outside the pH gradient depends on, e.g., the composition of the ampholytes (broad or narrow pH range) or on the composition of electrolyte solutions. The experiments performed in coated capillaries (with polyvinyl alcohol or with polyacrylamide) show that the amount and the injection length of the ampholytes influence the length of the pH gradient formed in the capillary. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“EN”> <标题类型=“main”>抽象</标题> >毛细管等电聚焦用质谱检测连字符，按顺序注射载体两种透射体和样品区，对于蛋白质表征具有高效。顺序注射方案的主要优点是具有pH范围的两种，其不应该覆盖样品组分的等电点，可用于分离。然后，该方法允许在不存在（留下pH梯度的物质）或在低水平的两种药物存在下（在pH梯度内迁移的物质）的情况下在线质谱检测分离的分析物。在pH梯度内或外部的物质的外观取决于大量的两种（宽或窄的pH范围）或电解质溶液组合物的组成。在涂覆的毛细血管（具有聚乙烯醇或聚丙烯酰胺）中进行的实验表明，两种分子的量和注射长度会影响毛细管中形成的pH梯度的长度。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第24期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Páger Csilla&option=202" target="_blank" rel="nofollow">Páger Csilla;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biherczová Nikoleta&option=202" target="_blank" rel="nofollow">Biherczová Nikoleta;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ligetvári Roland&option=202" target="_blank" rel="nofollow">Ligetvári Roland;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Berkics Balázs Viktor&option=202" target="_blank" rel="nofollow">Berkics Balázs Viktor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pongrácz Tamás&option=202" target="_blank" rel="nofollow">Pongrácz Tamás;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sándor Viktor&option=202" target="_blank" rel="nofollow">Sándor Viktor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bufa Anita&option=202" target="_blank" rel="nofollow">Bufa Anita;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Poór Viktória&option=202" target="_blank" rel="nofollow">Poór Viktória;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vojs Staňová Andrea&option=202" target="_blank" rel="nofollow">Vojs Staňová Andrea;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kilár Ferenc&option=202" target="_blank" rel="nofollow">Kilár Ferenc;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Department of Analytical ChemistryComenius University in BratislavaBratislava Slovak Republic;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> <p>Department of Analytical ChemistryComenius University in BratislavaBratislava Slovak Republic;</p> <p>Institute of Bioanalysis and Szentágothai Research CenterUniversity of PécsPécs Hungary;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=capillary isoelectric focusing&option=203" rel="nofollow">capillary isoelectric focusing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=carrier ampholytes&option=203" rel="nofollow">carrier ampholytes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=proteins&option=203" rel="nofollow">proteins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sequential injection&option=203" rel="nofollow">sequential injection;</a> </p> <div class="translation"> 机译：毛细管等电聚焦;载体两种;质谱;蛋白质;连续注射; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846534.html">Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Páger Csilla&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Páger Csilla,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biherczová Nikoleta&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Biherczová Nikoleta,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ligetvári Roland&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ligetvári Roland,</a> <a href="/journal-foreign-35800/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of separation science. .</a> <span>2017</span><span>,第24期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：顺序注射后毛细管等电聚焦分离蛋白质的高级在线质谱检测</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070404775364.html">Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling.</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang T&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang T,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma J&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ma J,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu S&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wu S,</a> <a href="/journal-foreign-22425/" target="_blank" rel="nofollow" class="tuijian_authcolor">Electrophoresis: The Official Journal of the International Electrophoresis Society .</a> <span>2011</span><span>,第20期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：毛细管等电聚焦，胰蛋白酶固定的酶微反应器和纳米反相液相色谱-质谱联用的集成平台，用于在线蛋白质谱分析。</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019463531.html">Separation and quantitation of milk whey proteins of close isoelectric points by on-line capillary isoelectric focusing-Electrospray ionization mass spectrometry in glycerol-water media</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lecoeur M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lecoeur M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gareil P.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gareil P.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Varenne A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Varenne A. </a> <a href="/journal-foreign-34102/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of chromatography, A: Including electrophoresis and other separation methods .</a> <span>2010</span><span>,第46期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：甘油-水介质在线毛细管等电聚焦-电喷雾电离质谱法分离和定量等电点附近的乳清蛋白</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-256173_thesis/0705011154610.html">Online Membrane-Assisted Buffer exchanger coupled with multijunction capillary Isoelectric focusing device enables fractionation of intact human plasma proteins by pl</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mohammad Pirmoradian&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mohammad Pirmoradian,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Juan Astorga-Wells&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Juan Astorga-Wells,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Roman A. Zubarev&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Roman A. Zubarev </a> <a href="/conference-foreign-256173/" target="_blank" rel="nofollow" class="tuijian_authcolor">ASMS Conference on Mass Spectrometry and Allied Topics .</a> <span>2015</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：与多结毛细管等电聚焦装置联接的在线膜辅助缓冲交换器能够通过PL分馏完整的人血浆蛋白</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061616966.html">Protein characterization by capillary isoelectric focusing electrophoresis, reversed phase liquid chromatography and mass spectrometry</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou, Feng.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhou, Feng. </a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过毛细管等电聚焦电泳，反相液相色谱和质谱法表征蛋白质</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040006000274.html">Femtomolar Concentration Detection Limit and Zeptomole Mass Detection Limit for Protein Separation by Capillary Isoelectric Focusing and Laser-induced Fluorescence Detection</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lauren M. Ramsay&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lauren M. Ramsay,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jane A. Dickerson&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jane A. Dickerson,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oluwatosin Dada&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Oluwatosin Dada,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：毛细管等电聚焦和激光诱导荧光检测分离蛋白质的飞摩尔浓度检测极限和分子质量检测极限</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000127980119.html">Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hai Dong Yu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hai Dong Yu,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Byungjoo Kim&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Byungjoo Kim,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dae-Ho Shin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dae-Ho Shin,</a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：开发用于在线毛细管等电聚焦 - 电喷雾质谱（CIEF-ESI-MS）的四通界面</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chemical-journal-chinese-universities_thesis/0201231607563.html">毛细管等电聚焦-毛细管区带电泳二维蛋白质分离平台的构建及其性能</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘和春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘和春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张维冰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张维冰</a> <span> <a href="/journal-cn-10958/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 高等学校化学学报 </a> </span> <span> . 2004</span><span>,第008期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230644470.html">毛细管等电聚焦/毛细管无胶筛分电泳二维蛋白质分离平台的构建</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘和春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘和春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨青&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨青</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2004</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chemical-journal-chinese-universities_thesis/0201231618151.html">用于蛋白质分析的毛细管等电聚焦-电喷雾质谱接口的改进</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许崇峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 许崇峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=宋浩威&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,宋浩威</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨芃原&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨芃原</a> <span> <a href="/journal-cn-10958/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 高等学校化学学报 </a> </span> <span> . 2002</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297527021.html">毛细管等电聚焦和电渗泵驱动聚焦区带分离蛋白质</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=舒馨&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 舒馨</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈令新&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈令新</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马继平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,马继平</a> <span> <a href="/journal-cn-57035/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析试验室 </a> </span> <span> . 2004</span><span>,第5期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230653629.html">毛细管柱表面鸡卵清蛋白快速改性并用于等电聚焦电泳分离蛋白质</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张维冰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张维冰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张玉奎&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张玉奎</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2003</span><span>,第009期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-58922_thesis/020222559473.html">毛细管等电聚焦(CIEF)/毛细管无胶筛分(CNGE)电泳二维蛋白质分离平台的构建</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘和春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘和春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨春&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨春</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张维冰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张维冰</a> <span> <a href="/conference-cn-58922/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十四次全国色谱学术报告会 </a> <span> <span> . 2003</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314251062.html">毛细管等电聚焦/加压毛细管电色谱多维联用及其在多肽蛋白质分离中的应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=魏娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 魏娟</a> <span> . 2011</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104784759.html">一种两步法毛细管电泳等电聚焦分离蛋白质的装置</a> <b>[P]</b> . <span> 中国专利： CN1590402A </span> <span> . 2005-03-09</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120302785.html">基于阵列毛细管等电聚焦的多维色谱－电泳分离与检测系统</a> <b>[P]</b> . <span> 中国专利： CN1316244C </span> <span> . 2007.05.16</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130434909242.html">ISOELECTRIC PARTICLES AND USES AS ELECTROPHORETIC TAGS FOR MULTIPLE ANALYTE DETECTION USING CAPILLARY ISOELECTRIC FOCUSING</a> <b>[P]</b> . <span> 外国专利：  WO2006087603A3 </span> <span> . 2007-04-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：等电粒子及其用作电泳标签的毛细管等电聚焦法在多种分析物检测中的应用 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130437195857.html">ISOELECTRIC PARTICLES AND USES AS ELECTROPHORETIC TAGS FOR MULTIPLE ANALYTE DETECTION USING CAPILLARY ISOELECTRIC FOCUSING</a> <b>[P]</b> . <span> 外国专利：  WO2006087603A2 </span> <span> . 2006-08-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：等电粒子及其用作电泳标签的毛细管等电聚焦法在多种分析物检测中的应用 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130460013779.html">Method of capillary isoelectric focusing of proteins and peptides with fraction collection for post-run analysis</a> <b>[P]</b> . <span> 外国专利：  EP0617048A1 </span> <span> . 1994-09-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：等分收集蛋白质和多肽的毛细管等电聚焦方法，用于运行后分析 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025846534','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025846534down" data-source="7," data-out-id="xbBLJ5jykZhpuVWFz8rbSrEYGZq79h0MEpy9XT9DPl7ovwP/mImtoPMexSNaoSg3," data-f-source-id="7" data-title="Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="0704025846534" id="docid"> <input type="hidden" value="35800" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/page.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/reference.js?v=5.8.0"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.8.0"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.8.0"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>