A sensitive, high‐throughput, and eco‐friendly analysis of daidzein and its valine carbamate prodrug in rat plasma by supercritical fluid chromatography with tandem mass spectrometry

Li Yingchao; Zhang Xingxing; Bai Xiaochen; Li Xiaoting; Jiang Qikun; Zhang Tianhong

摘要

Abstract > A valine carbamate prodrug of daidzein was synthesized to improve its bioavailability because of the poor solubility and low permeability of daidzein. To evaluate the pharmacokinetic behavior of the prodrug, a sensitive and high‐throughput method was developed and validated for the simultaneous determination of daidzein and its prodrug in rat plasma. The samples were extracted by ethyl acetate and then analyzed by a supercritical fluid chromatography with electrospray ionization tandem mass spectrometry method. The separation was achieved by an ACQUITY UPC 2 ? BEH 2‐EP column maintained at 40°C using carbon dioxide (≥99.99%) and methanol within 3.0?min by gradient elution. The mass transition ion pairs were m/z 254.8→136.7, 398.0→254.9, and 271.0→91.07 for daidzein, the prodrug, and genistein, respectively. The calibration curves were linear over the concentration ranges of 2–500 ( r ??0.997) and 10.0–5000.0?ng/mL ( r ??0.996) with lower limits of quantification of 2 and 10?ng/mL for daidzein and the prodrug, respectively. The intra‐ and interday accuracy and precision were within ±15% for all quality control samples. This developed method enabled high specificity, low cost, low solvent consumption, and a brief analysis time and was successfully applied to a bioavailability evaluation of daidzein and its carbamate prodrug. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</ title> >缬氨酸氨基甲酸盐氨基甲酸酯，因为溶解度差和低Daidzein的渗透性。为了评估前药的药代动力学行为，开发了敏感和高通量法，并验证了大鼠血浆中的DaIdzein及其前药的同时测定。通过乙酸乙酯萃取样品，然后通过超临界流体色谱法与电喷雾电离串联质谱法分析。通过ACCICE UPC <SUP> 2 </ SUP>实现分离？ BEH 2-EP柱在3.0℃下在3.0℃下在梯度洗脱中使用二氧化碳（≥99.99％）和甲醇。质量过渡离子对分别为Daidzinin，前药和Genistein的254.9和271.0→91.07分别为254.8→136.7,398.0→254.9和271.0→91.07。校准曲线在浓度范围内为2-500的浓度范围（ r =β0.997）和10.0-5000.0？ng / ml（ r </ i>？＆？0.996 ）分别为2和10μg/ ml的量化较少，分别用于Daidzein和前药。所有质量控制样品的内部和间隔准确性和精度都在±15％以内。这种开发方法使能高的特异性，成本低，溶剂消耗量低，以及简要的分析时间，并成功地应用于DaIdzein和氨基甲酸酯前药的生物利用度评价。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2018年第16期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Yingchao&option=202" target="_blank" rel="nofollow">Li Yingchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Xingxing&option=202" target="_blank" rel="nofollow">Zhang Xingxing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bai Xiaochen&option=202" target="_blank" rel="nofollow">Bai Xiaochen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Xiaoting&option=202" target="_blank" rel="nofollow">Li Xiaoting;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiang Qikun&option=202" target="_blank" rel="nofollow">Jiang Qikun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Tianhong&option=202" target="_blank" rel="nofollow">Zhang Tianhong;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang P. R. China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang P. R. China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang P. R. China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang P. R. China;</p> <p>Wuya College of InnovationShenyang Pharmaceutical UniversityShenyang P. R. China;</p> <p>Department of Pharmaceutical Analysis School of PharmacyShenyang Pharmaceutical UniversityShenyang P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=carbamate prodrugs&option=203" rel="nofollow">carbamate prodrugs;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=daidzein&option=203" rel="nofollow">daidzein;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=mass spectrometry&option=203" rel="nofollow">mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pharmacokinetics&option=203" rel="nofollow">pharmacokinetics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=supercritical fluid chromatography&option=203" rel="nofollow">supercritical fluid chromatography;</a> </p> <div class="translation"> 机译：氨基甲酸酯前药;Daidzein;质谱;药代动力学;超临界流体色谱; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846123.html">A sensitive, high‐throughput, and eco‐friendly analysis of daidzein 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<a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=彭子悦&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,彭子悦</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=江大森&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,江大森</a> <span> <a href="/journal-cn-14390/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 色谱 </a> </span> <span> . 2018</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-35540_thesis/020221315007.html">超临界流体色谱法在药物分析中的应用进展</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李修禄&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李修禄</a> <span> <a href="/conference-cn-35540/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 全国生化药物及药用动植物资源开发研究会议 </a> <span> <span> . 1994</span> </span> </div> </li> <li> <div> <b>7. 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