Isobaric tags for relative and absolute quantification-based proteomic analysis of defense responses triggered by the fungal pathogen Fusarium graminearum in wheat

摘要

Fusarium head blight (FHB) is a devastating disease worldwide that is predominantly caused by the fungal pathogen Fusarium graminearum. The aim of this work was to study differentially abundant protein species of near-isogenic lines A061-3 and A061-4 with the final goal of elucidating the molecular mechanisms of their differential resistance to F. graminearum. The objectives were accomplished using isobaric tags for relative and absolute quantification (iTRAQ) with mass spectrometry (MS). Lines A061-3 and A061-4 were resistant and susceptible to F. graminearum, respectively. At four post-inoculation points, 11,070 protein species were identified, of which 762 were differentially abundant. Gene Ontology enrichment analysis showed that most differentially abundant protein species participated in 18 biological processes after inoculation. Further analysis demonstrated that crucial metabolic pathways like plant-pathogen interaction had increased abundance. Real-time quantitative PCR (qRT-PCR) analysis revealed increased gene products of eight selected genes in plant pathogen interaction. This investigation provides a basic bioinformatics-based characterization of differentially abundant protein species during early stages against F. graminearum.