A Comparative Estimation of the Morphine Content of the Ayurvedic Formulation 'Kamini Vidrawan Ras' Using Validated High-Performance Thin-Layer Chromatography and High-Performance Liquid Chromatography Techniques

摘要

The separation and estimation of natural products by chromatographic techniques such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) are widely preferred. The present work reveals the quantification of morphine (a regulated narcotic agent) in the Ayurvedic formulation 'Kamini Vidrawan Ras' using reversed-phase HPLC (RP-HPLC). The results obtained herein were compared with the results of earlier reported work using HPTLC. The HPTLC separation of morphine was performed on an aluminum-backed layer of silica gel 60 F254 using ethyl acetate-methanol-ammonia solution as the mobile phase, while RP-HPLC was performed on Kromasil C8-column (150 mm x 4.6 mm, 5 mu m) using a mobile phase comprising of N-heptane sulfonate sodium salt-acetonitrile (70:30, v/v) at a flow rate of 1.5 mL min(-1). The International Conference on Harmonisation (ICH) guidelines were followed for validation of both the chromatographic methods. Both the developed chromatographic methods are simple, rapid, accurate, and sensitive.