Method for feeding a sample into an analysis branch of a liquid chromatography system, in particular a high-performance liquid chromatography system

摘要

Method for feeding a sample plug into an analysis branch (17) of a liquid chromatography unit, in particular high-performance liquid chromatography unit, a) in which a solvent (25) or a solvent mixture from at least one solvent branch (19) is conveyed as a volume flow Ȧ into the analysis branch (17), b) at least one sample from at least one sample branch (18) as a volume flow E in the analysis branch (17) within a predetermined time interval (Δ t = t ₄ - t ₁ ) is fed, characterized in that c) during the predetermined time interval (Δ t = t ₄ -t ₁ ) the volumetric flow Ȧ is reduced to an extent and as a result a sample in the form of a sample plug from a sample branch (18) is fed into the analysis branch (17) as a volumetric flow E, d) wherein a volume flow C resulting from the sum of the volume flows Ȧ and E remains substantially constant in the analysis branch (17) and e) the longitudinal mixing of the injected sample with the mobile phase is minimized on the way to a separation column, in which at least a sample branch (18) is a unidirectional acting valve with 2 switching positions in order to use the respective sample plug with particular accuracy can.