Physiological, anatomical and ultrastructural effects of aluminum onStyrax camporum, a native Cerrado woody species

摘要

Styrax camporumPohl. (Styracaceae) is a woody species that grows on acidic soils from the Brazilian savanna with high aluminum (Al) saturation (m% > 50%), where it accumulates similar to 1500 mg Al per kg dry leaves. Using nutrient solution, a previous study showed that 1480 mu M Al causes toxicity symptoms, which raises the question whether less than 1480 mu M Al could cause beneficial effects on this species. Here, we checked possible altered gas exchange rates, damage to organelles in root tips and the association between Al exposure and mitochondria occurrence in cells of root tips, once organic acids from Krebs cycle exuded by the roots of this species when exposed to Al have been recently evidenced. Five-month-old plants were grown in nutrient solution with 0, 740 and 1480 mu M Al for 90 days. Plants exposed to 1480 mu M Al showed less developed root system, reduced plant height and low gas exchange rates in relation to those exposed to 0 and 740 mu M Al, confirming that 1480 mu M Al is toxic toS. camporum. However, plants exposed to 0 and 740 mu M Al had similar number of leaves, plant height, root biomass, root length, total plant biomass and gas exchange rates, indicating that no beneficial effects from 740 mu M Al could be noted on this species. In plants exposed to 0 and 740 mu M Al, mitochondria were noted at the root tip, while at 1480 mu M Al these organelles were not evident due to the conspicuous vacuolation of root cells.S. camporumshows limited tolerance to Al in nutrient solution. In addition, this species is not dependent on Al to grow and develop because the plants grew well under 0 and 740 mu M Al.