Hepatic Histopathological Abnormalities in Rats Treated Topically with Para-Phenylene Diamine (PPD)

摘要

Drug and chemical mediated hepatotoxicity for wide numbers of chemicals has been recognized. The drug mediated hepatotoxicity and its evaluation is an important aspect in the development of drugs intended for therapeutic usages as well as chemicals used as food and cosmetic additive. Para-Phenylene Diamine (PPD), a widely used chemical in almost all hair dye formulation has been tested for its hepatotoxicity after 30 days continuous topical application in three different dosages (0,1, 2 and 3 mg kg"') in Sprague-Dawley rats. Serum biomarker (ALT, AST and ALP) of liver injury exhibit a dose dependent increases over control animals. Histopathological findings include centrilobular coagulative necrosis, periportal inflammation, fibrinous deposition, hemorrhages and increased accumulation of neutrophils within hepatic parenchyma. The PPD mediated hepatotoxicity is seems to be enhanced by increased accumulation of neutrophils.