Molecular mapping of stripe rust resistance geneYrH921in a wheat introgression line H921-11-1

摘要

Several novel stripe rust pathogen races emerging in the wheat-planting regions of China in recent years were virulent to a majority of the designated wheat seedling resistance genes. Therefore, it is of great significance to continuously select more new and valuable materials for enriching resistant sources diversity, pyramiding different excellent resistance genes and achieving durable resistance. In this study, a resistance gene, temporarily designated asYrH921, was identified from wheat-Psathyrostachys huashanica introgression line H921-11-1. Two hybrid populations, 160 F(2)plants and corresponding derived F(2:3)families, of the two parents about Mingxian169 as female and H921-11-1 as male, were used to evaluate stripe rust resistance in seedling stage and as a mapping population. At last, a genetic map which comprises of four simple sequence repeat (SSR) markers and six expressed sequence tag (EST) markers was constructed.YrH921was located on the long arm of chromosome 5A. Two closely linked EST-STS markers (BF483937andBF484913) were screened, and the genetic distance linked toYrH921was 3.0 and 4.3 cM, respectively. There was great value in research and production if the two closest markers were effectively used for marker-assisted selection ofYrH921in breeding.