Expression of recombinant Potato leafroll virus structural and non-structural proteins for antibody production.

Plchova H.; Moravec T.; Dedic P.; Cerovska N.

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Expression of recombinant Potato leafroll virus structural and non-structural proteins for antibody production.

机译：重组的表达马铃薯乳酸乳酸乳酸乳酸乳酸病毒抗体生产的结构和非结构蛋白。

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Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp_43-616 and RdRp_304-537 were chosen for expression. A high level of CP and RdRp_304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.

机译：载体PMPM-A4 OMEGA和含有6倍HIS-TAC序列的PQE-30和PET-45B（+）用于表达马铃薯乳蛋草病毒（PLRV）结构和非结构蛋白我>大肠杆菌。选择外壳蛋白质（CP）和RNA依赖性RNA聚合酶（RDRP）-FRAGENTS RDRP _{43-616 和RDRP _{304-537 表达。仅在使用PET-45B（+）载体和 e的表达系统中获得高水平的CP和RDRP _{304-537 。 COLI Rosetta-GAMI 2（DE3）细胞。纯化后，他标记的PLRV蛋白被用于兔免疫。}}}

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来源
《Journal of Phytopathology》 |2011年第2期|共3页
作者
Plchova H.; Moravec T.; Dedic P.; Cerovska N.;
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作者单位

Institute of Experimental Botany v. v. i. Academy of Sciences of the Czech Republic Na Karlovce 1a 160 00 Prague 6 Czech Republic;

Institute of Experimental Botany v. v. i. Academy of Sciences of the Czech Republic Na Karlovce 1a 160 00 Prague 6 Czech Republic;

Potato Research Institute Havlickuv Brod Dobrovskeho 2366 580 01 Havlickuv Brod Czech Republic;

Institute of Experimental Botany v. v. i. Academy of Sciences of the Czech Republic Na Karlovce 1a 160 00 Prague 6 Czech Republic;

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正文语种 eng
中图分类植物生理学;
关键词
antibodies; antigens; coat proteins; immunization; plant pathogens; plant viruses; potatoes; purification; RNA; RNA polymerase; vectors; Escherichia; Escherichia coli; Potato leafroll virus; rabbits; Solanum tuberosum; Escherichia; Enterobacteriaceae; Enterobacteriales; Gammaproteobacteria; Proteobacteria; Bacteria; prokaryotes; Polerovirus; Luteoviridae; positive-sense ssRNA viruses; ssRNA viruses; RNA viruses; viruses; Leporidae; Lagomorpha; mammals.vertebrates; Chordata; animals; small mammals; eukaryotes; Solanum; Solanaceae; Solanales; dicotyledons; angiosperms; Spermatophyta; plants; antigenicity; bacterium; capsid proteins; immune sensitization; immunogens; phytopathogens; ribonucleic acid; RNA nucleotidyltransferase; viruses of plants;

机译：抗体;抗原;涂层蛋白;免疫;植物病毒;土豆;纯化;RNA;RNA聚合酶;vectorichia;raberichia;马铃薯;兔子;芦荟菌;ranum therosum;encherichia;肠杆菌;肠杆菌;细菌;原核生物;挥发性病毒;阳性感觉;ssRNA病毒;RNA病毒;病毒;LagoMorpha;哺乳动物;脊髓属;动物;小哺乳动物;Solanum;solanaLes;茄属植物;鞘糖蛋白;嗜睡症;子宫植物;嗜睡症;嗜睡症;嗜睡症;嗜睡症;癫痫菌;植物;抗原;细菌;衣壳蛋白;免疫敏化;免疫原;植物藻酸;核糖核酸;RNA核苷酸转移酶;植物病毒;

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1. Expression of recombinant Potato leafroll virus structural and non-structural proteins for antibody production. [J] . Plchova H., Moravec T., Dedic P., Journal of Phytopathology . 2011,第2期

机译：重组的表达马铃薯乳酸乳酸乳酸乳酸乳酸病毒抗体生产的结构和非结构蛋白。
2. Production of Polyclonal Antibodies to the Recombinant Potato virus MTI Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein [J] . Cerovska N, Moravec T, Plchova H, Journal of Phytopathology . 2012,第5期

机译：重组马铃薯病毒MTI多克隆抗体的生产重组马铃薯病毒M（PVM）非结构性三联基因阻断蛋白1和外壳蛋白的多克隆抗体的生产
3. Production of polyclonal antibodies to a recombinant Potato mop-top virus non-structural triple gene block protein 1. [J] . Cerovska N, Filigarova M, Pecenkova T Journal of Phytopathology . 2006,第7a8期

机译：重组马铃薯拖把病毒非结构性三联基因阻断蛋白1的多克隆抗体的产生。
4. Expression and Purification of Non-Structural Protein NS1 of Human Bocavirus and Generation of Its Antibody [C] . Sun Bin, Yue Pengying, Li Jingjing, 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：人博卡病毒非结构蛋白NS1的表达，纯化及其抗体的产生
5. Antibody response to non-structural proteins: Implications for diagnostic detection and differentiation of type 1 and type 2 porcine reproductive and respiratory syndrome virus. [D] . Brown, Elizabeth Marie. 2008

机译：抗体对非结构蛋白的应答：对1型和2型猪繁殖与呼吸综合征病毒的诊断检测和分化的意义。
6. Expression purification and characterization of recombinant severe acute respiratory syndrome coronavirus non-structural protein 1 [O] . Kimberly Brucz, Zachary J. Miknis, L. Wayne Schultz, -1

机译：重组重症急性呼吸综合征冠状病毒非结构蛋白1的表达纯化及鉴定
7. Production of Polyclonal Antibodies to a Recombinant Potato Mop-top Virus Non-structural Triple Gene Block Protein 1 [O] . N. Cerovska ˇ ˇ, M. Filigarova ́, T. Pecenkova ˇ 2005

机译：生产重组马铃薯拖把病毒非结构三基因块蛋白1的多克隆抗体
8. Dengue-1 Virus Envelope Glycoprotein Gene Expressed in Recombinant BaculovirusElicits Virus-Neutralizing Antibody in Mice and Protects them from Virus Challenge. (Reannouncement with New Availability Information) [R] . Putnak, R., Feighny, R., Burrous, J., 1991

机译：登革1病毒包膜糖蛋白基因在重组杆状病毒中表达，在小鼠中引发病毒中和抗体并保护它们免受病毒攻击。（重新公布新的可用性信息）

Expression of recombinant Potato leafroll virus structural and non-structural proteins for antibody production.

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