A single tube system for the detection of Mycobacterium tuberculosis DNA using gold nanoparticles based FRET assay

摘要

The global combat against MTB is limited by challenges in accurate affordable detection. In this study, a rapid, affordable, single tube system for detection of unamplified MTB16s rDNA was developed. Utilizing a AuNP based FRET system, this assay achieved a sensitivity and specificity of 98.6% and 90% respectively. Highlights ? AuNP-FRET assay was developed for the detection of MTB DNA. ? The developed assay has a sensitivity of 98.6% and specificity of 90%. ? The assay is able to differentiate MTB from nontuberculous mycobacteria (NTM). ? The assay does not require DNA amplification and utilizes a single tube. ? Assay turnaround time is 15min.